ABSTRACT
PURPOSE: To evaluate the effects of angico bark extract (Anadenanthera colubrina var. cebil) in the healing process of the skin of rats. METHODS: Twenty adult male rats were divided into four groups of five animals each, according to the respective postoperative days, as follow: G4, G7, G14 and G21. Each group received two incisions on skin and subcutaneous tissue in the right and left antimere of the thoracic region, separated by a distance of 2 cm. The right lesion was treated daily with saline and the left with the angico alcoholic extract (5%). At the end of each experimental period, the animals were euthanized and fragments of the wound area with the edges were removed, fixed in 10% formaldehyde solution and processed for paraffin embedding. Histological sections (5 μm of thickness) were stained with hematoxylin and eosin (HE), Gomori trichromic and picrosisirus red for morphological and morphometric analyses. Statistical analysis was done by ANOVA and Tukey-Kramer test (p<0.05). RESULTS: Morphological analysis showed larger fibroblasts and a higher concentration of collagen fibers in skyn wounds treated with the angico extract. Morphometric analysis demonstrated a significant increase in the number of fibroblasts at 7th and collagen in 7th and 14th days (p<0.01) in wounds treated with the angico extract. CONCLUSION: The angico alcoholic extract (Anadenanthera colubrina var. cebil) induces the acceleration of wound healing in skin wounds of rats. .
Subject(s)
Animals , Male , Rats , Fabaceae/chemistry , Phytotherapy/methods , Plant Extracts/therapeutic use , Skin/drug effects , Skin/pathology , Wound Healing/drug effects , Cell Count , Collagen/analysis , Fibroblasts/drug effects , Paraffin Embedding , Postoperative Period , Reproducibility of Results , Skin/injuries , Subcutaneous Tissue/drug effects , Subcutaneous Tissue/injuries , Subcutaneous Tissue/pathology , Time Factors , Treatment OutcomeABSTRACT
O potencial visual evocado por padrão reverso (PVE-PR) é um método objetivo, não invasivo e estabelecido para as investigações funcionais. Este trabalho teve como objetivo a obtenção de dados do PVE-PR com tabuleiro de xadrez em gatos domésticos adultos, além de verificar a influência de diferentes ângulos visuais sobre os picos de tempo implícito e amplitude das ondas N75 e P100. Foram utilizados 13 gatos adultos (2-4 anos de idade), de ambos os sexos. Cada animal foi posicionado 100cm de distância de um monitor de vídeo situado a 17,2º. O estímulo gerado para a formação dos PVEs foi uma onda "quadrada" com a frequência de 6 Hz, apresentados em cinco frequências espaciais (16', 32', 64', 130' e 260'de arco). Os valores médios dos tempos implícitos (ms) e amplitudes (µV) de P100 não foram diferentes estatisticamente e nem estavam correlacionados (r=0,067). Com a mudança de tamanho do ângulo de estímulo apresentado, os picos de tempo implícito e amplitude do P100 apresentaram uma discreta variação. Aos 32' de arco, a amplitude de P100 atingiu seu pico máximo, com 14,94±1,13µV (média±erro padrão da média). N75 não mostrou nenhuma variação de amplitude. Com 16' de arco, o tempo implícito de N75 diminuiu, não havendo correlação com os valores de amplitude (r=0,26). O ângulo visual que desencadeou um maior pico de amplitude e tempo implícito constante para P100, foi o de 32' de arco. Em gatos adultos, um PVE-PR pode ser obtido sem o uso de midriáticos e sedativos, com estímulos subtendidos entre ângulos grandes como 64' e pequenos como 16'.
The pattern reversal visual evoked potential (PR-VEP) is an objective method, stablished for noninvasive functional investigations. This research aimed to obtain data from the PR-VEP with checkerboard in adult domestic cats and the influence of different visual angles on the peak latency and implicit time of waves N75 and P100. It was used 13 adult cats (2-4 years old), of both sexes. Each animal was positioned 100 cm away from a video monitor located at 17.2º. The stimulus generated for the formation of wave VEPs was "square" with the frequency of 6Hz, presented in five spatial frequencies (16 ', 32', 64 ', 130' and 260 'of arc). Mean values of implicit times (ms) and amplitudes (µvolts) of P100 were not statistically different and were not correlated (r=0.067). With the change in size of the angle of stimulus presented, the peak implicit time and amplitude of P100 showed a slight variation. At 32 'of arc amplitude of P100 reached its peak with 14,94±1,13µV (mean±SEM). N75 showed no variation in amplitude. With 16'of arc the implicit time N75 decreased and no correlation with amplitude values was observed (r=0,26). The visual angle that triggered higher peak amplitude and implicit time constant for P100, was 32'of arc. In adult cats, PR-VEP can be obtained without mydriatic and sedatives, with stimuli subtending angles between large as 64' and small as 16 '.